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Freshly isolated monocytes and neutrophils were seeded in a polypropylene 96-well plate (0.2 × 10 6 cells per well) and washed twice with flow cytometry staining buffer (PBS containing 0.5% endotoxin-free BSA, 2 mM EDTA, and 0.1% NaN 3 [Merck Millipore, 1687])
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